Pharmacological ZO-1 agonist treatment attenuates ammonia nitrogen stress-induced mucosal inflammation and intestinal barrier failure in Leiocassis longirostris

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Ammonia nitrogen exposure induces inflammatory cell infiltration and disrupts the intestinal epithelial barrier in Leiocassis longirostris.
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Despite upregulated transcripts of tight junction components, protein assembly at the apical membrane is severely disrupted under ammonia stress.
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Pharmacological stabilization of ZO-1 reduces mucosal inflammatory damage and restores tight junction protein localization and barrier function.
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The study identifies a pattern of ineffective transcriptional compensation in response to ammonia-induced mucosal dysregulation.
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Stabilizing ZO-1 may strengthen intestinal mucosal resilience, providing insights for sustainable aquaculture practices.

Frontiers In Immunology

by Senyue Liu, Yang Feng, Chengyan Mou, Lu Zhang, Yuanliang Duan, Zhipeng Huang, Han Zhao, Jian Zhou, Jun Du, Qiang Li, Yongqiang Deng
June 8, 2026

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