Integrated single-cell and bulk RNA sequencing analyses identify a myeloid state-related gene signature for molecular subtyping in stomach adenocarcinoma - Report - MDSpire
Advertisement
Integrated single-cell and bulk RNA sequencing analyses identify a myeloid state-related gene signature for molecular subtyping in stomach adenocarcinoma
Clinical Report: Combined Single-Cell and Bulk RNA Sequencing Reveals a Gene Signature Related to Myeloid States for Molecular Classification in Gastric Adenocarcinoma
Overview
This study identifies a myeloid state-related prognostic gene signature in gastric adenocarcinoma (STAD) and establishes a molecular classification framework (STAD-MSC) that stratifies patients based on immune infiltration.
Background
Gastric adenocarcinoma (STAD) is a highly heterogeneous malignancy with a poor prognosis, particularly in advanced stages. The tumor microenvironment, particularly the role of myeloid cells, is crucial in modulating tumor behavior. Current classification methods based on histopathology are insufficient, necessitating novel approaches that consider the dynamic interactions within the tumor microenvironment.
Data Highlights
Finding
Details
MSRPGs Identified
32 myeloid state-related prognostic genes across five distinct states
Patient Subtypes
Three subtypes: low immune infiltration (LI-STAD), moderate immune infiltration (MI-STAD), high immune infiltration (HI-STAD)
Survival Analysis
HI-STAD subtype showed the poorest overall survival (global log-rank p = 0.018)
5-Gene Risk Model
Evaluated in a 355-patient validation cohort
Protein Classifier
Three-protein classifier (NNMT/AXL/COL1A1) stratified a 70-patient cohort with significant differences in OS and PFS
