Serum and serum-derived extracellular vesicle microRNA signatures linked to neurodevelopmental processes in central precocious puberty - Report - MDSpire
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Serum and serum-derived extracellular vesicle microRNA signatures linked to neurodevelopmental processes in central precocious puberty
MicroRNA Profiles in Serum and Extracellular Vesicles Associated with Neurodevelopmental Changes in Central Precocious Puberty
Overview
This study identifies distinct microRNA profiles in serum and extracellular vesicles from female patients with central precocious puberty (CPP).
Background
Central precocious puberty (CPP) is characterized by the premature activation of the hypothalamic-pituitary-gonadal (HPG) axis, primarily affecting girls. Understanding the molecular mechanisms behind CPP is crucial.
Data Highlights
MicroRNA
Expression Change
miR-125a-5p
Decreased
miR-125b-5p
Decreased
miR-99b-5p
Decreased
miR-148a-3p
Increased in EVs
Key Findings
Ten microRNAs were identified with significantly altered expression levels in CPP patients.
Pathway enrichment analysis indicated involvement in neurodevelopment, growth regulation, and cellular maturation.
RT-qPCR validation confirmed decreased levels of miR-125a-5p, miR-125b-5p, and miR-99b-5p in CPP serum samples.
All selected microRNAs were detectable in serum-derived extracellular vesicles.
miR-148a-3p showed a significant increase specifically in the EV-associated fraction of CPP samples.
Clinical Implications
The findings indicate altered microRNA profiles in CPP patients.
Conclusion
This research provides insights into the microRNA landscape associated with central precocious puberty.
by Maria Morrou, Vassos Neocleous, Meropi Toumba, Marios Tomazou, Louiza Potamiti, Maria Zanti, Kyriaki Michailidou, Fotios Mpekris, George M. Spyrou, Andreas Protopapas, Elena Sotiriou, Michalis Iasonides, Nicos Skordis, Pavlos Fanis, Leonidas A. Phylactou
