Molecular mechanisms of suxiao jiuxin pills in ameliorating post-acute myocardial infarction inflammatory response: a combined network pharmacology, Mendelian randomization, and experimental validation study - Report - MDSpire
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Molecular mechanisms of suxiao jiuxin pills in ameliorating post-acute myocardial infarction inflammatory response: a combined network pharmacology, Mendelian randomization, and experimental validation study
Clinical Report: Investigating the Molecular Pathways of Suxiao Jiuxin Pill
Overview
This study explores the molecular mechanisms of Suxiao Jiuxin Pill (SJP) in reducing inflammatory responses following acute myocardial infarction (AMI). Key findings include the identification of two target genes, NAMPT and FOS, which are significantly upregulated in AMI and associated with immune cell responses.
Background
Acute myocardial infarction (AMI) is a leading cause of mortality worldwide, necessitating the exploration of novel therapeutic strategies. Traditional Chinese medicine, including Suxiao Jiuxin Pill (SJP), has gained attention for its potential in managing cardiovascular diseases. Understanding the molecular pathways involved in SJP's action is essential.
Data Highlights
Finding
Details
DE-TGs Identified
44 differentially expressed target genes (DE-TGs) from 689 DEGs and 969 predicted target genes.
Key Genes
NAMPT and FOS showed markedly upregulated expression in AMI samples.
Correlation with Immune Cells
NAMPT exhibited a strong positive correlation with neutrophils (cor = 0.65).
Molecular Docking
NAMPT bound to oleic acid (−5.9 kcal/mol) and FOS bound to pentadecanol (−5.4 kcal/mol).
Cardiac Function Improvement
SJP ameliorated cardiac function and reduced inflammatory responses in MI rats.
Key Findings
Identification of 44 DE-TGs relevant to SJP's action in AMI.
NAMPT and FOS are key target genes with significant upregulation in AMI.
NAMPT shows a strong positive correlation with neutrophils.
Molecular docking indicates specific binding affinities of NAMPT and FOS.
SJP improves cardiac function and reduces inflammation in a rat MI model.
Clinical Implications
The findings provide insights into the roles of NAMPT and FOS in the context of AMI.
Conclusion
This study provides insights into the molecular mechanisms of SJP in AMI.
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