Clinical Report: CYP1B1 as a Potential Target for Orientin in Low Back Pain

Overview

This study identifies CYP1B1 as a candidate hub gene for orientin in treating low back pain associated with intervertebral disc degeneration (IDD). The findings indicate that orientin alleviated IL-1β induced cell viability decrease and down-regulated CYP1B1 expression.

Background

Low back pain is a prevalent condition often linked to intervertebral disc degeneration (IDD). Understanding the molecular mechanisms and potential therapeutic targets is crucial for developing effective treatments. Orientin is a natural bioactive agent that has been studied for its effects in pain management.

Data Highlights

This study utilized network pharmacology, machine learning, and various validation techniques to explore the relationship between orientin and IDD-associated low back pain.

Key Findings

• 368 genes were identified as overlapping between orientin and IDD-associated low back pain.
• CYP1B1 was identified as a candidate hub gene with an AUC of 0.95 for diagnostic performance.
• No correlation was found between CYP1B1 and immune cell infiltration levels after correction.
• Molecular docking showed favorable binding energies of orientin to CYP1B1.
• scRNA-seq analysis indicated significant upregulation of CYP1B1 in diseased annulus fibrosus fibroblasts.
• Orientin alleviated IL-1β induced decreases in cell viability and down-regulated CYP1B1 expression.

Clinical Implications

The identification of CYP1B1 as a target for orientin is based on the findings of this study.

Conclusion

CYP1B1 is identified as a potential therapeutic target for orientin in the context of IDD-associated low back pain.

Related Resources & Content

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3. Frontiers in Immunology, 2026 -- Revisiting Intervertebral Disc Degeneration: Lipid Metabolism Disorders as Overlooked Contributors to Complex Pathology
4. Biology of intervertebral disc degeneration - ScienceDirect, 2025
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7. Bone marrow concentrate intradiscal injection for chronic discogenic low back pain: A double-blind randomized sham-controlled trial - PMC
8. Magnetic Resonance Spectroscopy Lipids Peak May Serve as a Potential Biomarker for Back Pain in Intervertebral Disc Degeneration: An Integrative Metabolomics and Proteomics Study Investigating the Role of the Lipid Droplets-Interleukin-17 Inflammatory Axis - PubMed