Casein hydrolysate promotes intestinal repair in adult mice with Salmonella enteritis through a lactate-GPR81-Wnt3A axis in intestinal stem cell niche - Report - MDSpire

Casein hydrolysate promotes intestinal repair in adult mice with Salmonella enteritis through a lactate-GPR81-Wnt3A axis in intestinal stem cell niche

  • By

  • Jianbo Cheng

  • Jing Li

  • Yuying Bai

  • Shi Wang

  • Lirui Sun

  • June 15, 2026

  • 0 min

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Casein Hydrolysate Enhances Intestinal Healing in Adult Mice with Salmonella Enteritis

Overview

Casein enzymatic hydrolysate (CEH) significantly improves survival and intestinal healing in mice with Salmonella enteritis.

Background

Salmonella enterica serovar Typhimurium is a significant enteric pathogen that leads to severe intestinal damage.

Data Highlights

ParameterControlModelModel + CEH
Survival Rate100%50%80%
Inflammation (TNF-α)LowHighModerate
Stem Cell Activity (LGR5+)NormalDecreasedIncreased

Key Findings

  • CEH administration improved survival and reduced clinical disease severity in mice.
  • CEH preserved mucosal architecture and restored barrier proteins.
  • CEH enhanced intestinal stem cell regeneration.
  • CEH reshaped gut microbiota.
  • Pharmacological blockade of GPR81 or Wnt signaling negated the effects of CEH.

Clinical Implications

The findings suggest that CEH could be a beneficial nutritional strategy for managing intestinal injuries caused by infections. Understanding the lactate-GPR81-Wnt3A pathway may inform future therapeutic interventions aimed at enhancing intestinal healing.

Conclusion

CEH demonstrates significant potential in alleviating Salmonella-induced enteritis through a specific signaling pathway, highlighting its role in promoting intestinal regeneration.

Related Resources & Content

  1. Models for Assessing Intestinal Anastomosis Healing in Experimental Colitis, Springer, 2021 -- Models for Assessing Intestinal Anastomosis Healing in Experimental Colitis
  2. Bifidobacterium bifidum CIP-01 Reduces Liver Disease Linked to Metabolic Dysfunction Induced by High-Alcohol-Producing Klebsiella pneumoniae, Journal of Gastroenterology, 2025 -- Bifidobacterium bifidum CIP-01 Reduces Liver Disease Linked to Metabolic Dysfunction Induced by High-Alcohol-Producing Klebsiella pneumoniae
  3. Recent Strategies for Enhancing Healing of Gastrointestinal Anastomoses: A Review of Experimental and Clinical Insights, Springer, 2020 -- Recent Strategies for Enhancing Healing of Gastrointestinal Anastomoses: A Review of Experimental and Clinical Insights
  4. Salmonella Infection | Salmonella Infection (Salmonellosis) | CDC, CDC, 2025 -- Salmonella Infection | Salmonella Infection (Salmonellosis) | CDC
  5. Journal of Gastroenterology — Resistant starch inhibits glycolysis via HK2 and mitigates colon tumor development induced by high-fructose corn syrup
  6. Guidelines for the Prevention and Treatment of Opportunistic Infections in Adults and Adolescents
  7. Salmonella Infection | Salmonella Infection (Salmonellosis) | CDC
  8. Lactate alleviates intestinal barrier injury in weaned piglets via activation of the Wnt/β-catenin pathway and promotion of intestinal epithelial cell proliferation - PubMed

Original Source(s)

Casein hydrolysate promotes intestinal repair in adult mice with Salmonella enteritis through a lactate-GPR81-Wnt3A axis in intestinal stem cell niche

  • by Jianbo Cheng, Jing Li, Yuying Bai, Shi Wang, Lirui Sun

  • June 15, 2026

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