Clinical Report: Submicron Emulsion of Tea Saponin and Soybean Oil Enhances Long-lasting Immune Protection Against Pasteurella multocida
Overview
This study demonstrates that a tea saponin-soybean oil submicron emulsion (TS-Pm) significantly enhances the immune response of an inactivated Pasteurella multocida vaccine. TS-Pm improved antibody persistence and survival rates in mice following lethal bacterial challenge.
Background
Pasteurella multocida is a significant pathogen in veterinary medicine, causing respiratory and systemic infections. Current inactivated vaccines often provide limited protection due to weak immune activation and short-lived antibody responses.
Data Highlights
Parameter
TS-Pm
Alum-Pm
Survival Rate after Lethal Challenge
90%
60%
Antibody Response (Pm-specific IgG)
Higher
Lower
Particle Size
~300 nm
N/A
Storage Stability
6 months at 4 °C
N/A
Key Findings
TS-Pm formed a stable oil-in-water submicron emulsion with a particle size of approximately 300 nm.
Increased antigen uptake and pro-inflammatory cytokine production were observed in RAW264.7 macrophages treated with TS-Pm.
TS-Pm maintained higher Pm-specific IgG responses compared to the alum-adjuvanted vaccine.
After lethal challenge, TS-Pm improved survival rates to 90% and reduced pulmonary bacterial burden.
Draining lymph-node proteomics indicated changes associated with antigen processing and immune-cell trafficking.
Clinical Implications
The findings indicate that TS-Pm may enhance humoral and cellular immune responses.
Conclusion
The tea saponin-soybean oil submicron emulsion enhances the efficacy of inactivated P. multocida vaccines.
