Clinical Report: Comprehensive Evaluation of GEO Data on Microbial Communities

Overview

This study characterizes gut microbiota dysbiosis in colorectal cancer (CRC) and its association with the tumor immune microenvironment. Key findings include significant alterations in microbial diversity and specific bacterial genera linked to immune cell proportions.

Background

Colorectal cancer (CRC) is a leading cause of cancer-related morbidity and mortality worldwide. Recent research highlights the role of gut microbiota dysbiosis in CRC development.

Data Highlights

Key Findings

• CRC patients showed significantly reduced α diversity and altered microbial community structure.
• Seven key differentially abundant genera were identified, including pathogenic bacteria Fusobacterium nucleatum and Bacteroides fragilis.
• Beneficial bacteria such as Akkermansia muciniphila and Roseburia intestinalis were depleted in CRC tissues.
• Pathogenic bacteria positively correlated with myeloid cells and negatively with B-cells.
• KEGG pathway analysis revealed significant enrichment in immune signaling pathways.
• Six out of seven core bacterial genera demonstrated consistent abundance changes in an independent validation cohort.

Clinical Implications

The findings indicate an association between gut microbiota dysbiosis and the tumor microenvironment in CRC.

Conclusion

This study provides a comprehensive analysis of gut microbiota dysbiosis in CRC and its implications for the tumor immune microenvironment.

Related Resources & Content

1. Frontiers in Oncology, 2026 -- The gut microbiota in colorectal cancer: role in cytokine regulation, intestinal immune barrier dysfunction
2. Frontiers in Immunology, 2026 -- Multidimensional exploration of the relationship between gut microbiota and colorectal cancer: focus on clinical tumorigenesis and treatment
3. Frontiers in Immunology, 2026 -- Spatial immune archetypes in gastric and colorectal cancer: a proposed conceptual framework for immunotherapy resistance and therapeutic remodeling
4. Colorectal Cancer | American Cancer Society -- Screening Guidelines
5. Metastatic colorectal cancer: ESMO Clinical Practice Guideline for diagnosis, treatment and follow-up - PubMed
6. Gastric Cancer — Exploring the Connection Between the Gastric Cancer Microbiome and Clinical-Pathological Characteristics: Insights from the 100,000 Genomes Project and The Cancer Genome Atlas
7. Microbiome Modulation for the Treatment of Solid Neoplasms | Journal of Clinical Oncology
8. The immune microenvironment of colorectal cancer | Nature Reviews Cancer
9. Gut microbiome signatures in colorectal neoplasia: a cross-sectional study across neoplasia stages and subtypes
10. Colorectal Cancer | American Cancer Society
11. Metastatic colorectal cancer: ESMO Clinical Practice Guideline for diagnosis, treatment and follow-up - PubMed
12. Meta-analysis reveals microbiome signatures for colorectal cancer that are universal across age groups and sequencing methods - PubMed
13. Prognostic impact of Fusobacterium nucleatum on survival in colorectal cancer: A systematic review and meta-analysis - PubMed
14. Gut microbiota-associated predictors as biomarkers of neoadjuvant treatment response in rectal cancer-a systematic review | British Journal of Cancer