White matter microdissection of the lateral aspect of the brain: 2-dimensional video demonstration

Category	Detail
Condition	Anatomical study and dissection of lateral brain white matter
Key Mechanisms	Klingler fixation protocol involving formalin fixation, freezing, thawing, and microscopic dissection to reveal white matter fiber tracts
Target Population	Neurosurgeons and neuroanatomists interested in brain white matter anatomy
Care Setting	Anatomy laboratories and neurosurgical training environments

Klingler’s method remains the reference standard for white matter dissection due to reproducibility and accessibility.
Freezing formalin-fixed brain specimens improves clarity and precision of fiber tract visualization.
Microscopic visualization enhances the dissection process, allowing detailed study of white matter bundles.

Use detailed sulcal and gyral anatomy identification as landmarks for white matter dissection.

Fix brain specimens in 10% formalin for at least two months with suspension to avoid deformation.
Freeze specimens at −10 to −15 °C for at least one week to improve fiber tract separation.
Thaw specimens in water and carefully remove pia mater, arachnoid, and blood vessels before dissection.
Perform dissection under microscopic visualization using appropriate tools (wooden spatulas, forceps, scalpel).
Maintain specimen hydration with regular irrigation during dissection.

Store dissected brain specimens in 5% formalin between sessions.
Refrigerate specimens for at least 12 hours before resuming dissection if postponed for a month or longer.

Potential deformation of brain tissue if not properly suspended during fixation.
Loss of anatomical detail if freezing or thawing protocols are not adequately followed.

Not applicable; technique pertains to anatomical specimen preparation and dissection.

Not applicable.

Begin dissection with thorough study of sulcal and gyral anatomy to identify key landmarks.
Apply the double-C decortication scheme to expose U-fibers effectively.
Use microscopic visualization throughout dissection to enhance precision and safety.
Employ a combination of wooden spatulas, forceps, scalpel, and irrigation to optimize dissection quality.
Extend freezing duration when possible to improve fiber tract separation.

Clinical Scorecard: Lateral Brain White Matter Microdissection: A Two-Dimensional Video Overview