Novel tracking system aligns pCLE probe data with HSI images to overcome navigation challenges and improve surgical accuracy
Guideline-Based Recommendations
Diagnosis
Use HSI to noninvasively differentiate tissue types and identify tumor regions intraoperatively
Employ pCLE for in vivo cellular-level visualization of tumor margins to guide resection
Management
Integrate HSI and pCLE imaging modalities into the surgical workflow to enhance tumor margin delineation
Utilize real-time tracking of pCLE probe to correlate microscopic findings with wide-field HSI data
Monitoring & Follow-up
Perform white-balance calibration for RGB and HSI modalities to ensure consistent imaging quality
Continuously capture and align multimodal images during surgery to monitor tumor resection progress
Risks
Avoid excessive resection in functionally active brain areas to prevent postoperative disability
Be aware of limitations of individual modalities: pCLE’s small field of view and HSI’s spectral range constraints
Patient & Prescribing Data
Patients with brain tumors requiring surgical resection, particularly gliomas and meningiomas
Multimodal imaging combining HSI and pCLE can improve accuracy of tumor margin identification, potentially leading to more complete resections and better functional outcomes
Clinical Best Practices
Calibrate imaging devices prior to surgery to ensure accurate color and spectral data
Use the operating microscope as the reference frame for integrating multimodal imaging data
Employ high-frame-rate RGB imaging to track pCLE probe position and align with HSI images
Leverage complementary strengths of HSI and pCLE for comprehensive tumor margin assessment
Maintain surgeon workflow by integrating imaging modalities without disrupting surgical procedures
