Optimizing the sensitivity of detection of respiratory syncytial virus infections in longitudinal studies using the combination of weekly sample testing and biannual serology - Summary - MDSpire
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Optimizing the sensitivity of detection of respiratory syncytial virus infections in longitudinal studies using the combination of weekly sample testing and biannual serology
To improve detection sensitivity of RSV infections in a birth cohort through a combination of weekly nasal sampling and biannual serological assessments, enhancing the understanding of disease burden.
Key Findings:
Cumulative incidence of RSV increased from 49% (RT-qPCR only) to 75% (combined methods, P < .001), indicating a significant improvement in detection.
Incidence rate rose from 0.33 to 0.71 infections/child-year (P < .001) when combining RT-qPCR and serology, highlighting the effectiveness of the combined approach.
Antibody thresholds identified were log10 change in IgG > 0.32 or IgA > 0.20, which were statistically significant indicators of RSV infections.
Interpretation:
The combination of RT-qPCR and serological assessments significantly enhances the detection of RSV infections, capturing a broader incidence than RT-qPCR alone.
Limitations:
Potential selection bias due to varying adherence levels among participants, which may skew the incidence rates reported.
Serological assays cannot date infections, limiting assessment of symptomatology and the understanding of disease progression.
Conclusion:
Integrating weekly sampling with serological assessments provides a more comprehensive understanding of RSV infections, crucial for evaluating vaccine effectiveness and disease burden, and suggests directions for future research.
by Shannon C Conrey, Daniel C Payne, Maria Deza Leon, Monica Epperson, Melissa M Coughlin, Allison R Burrell, Claire P Mattison, Rachel M Burke, Julia M Baker, Natalie J Thornburg, Meredith L McMorrow, Mary Allen Staat, Mary Allen Staat, Ardythe L Morrow
