Objective:

To conduct a systematic literature search to identify rodent studies reporting significant modulation of NAD+ levels in adipose tissue and associated changes in gene expression.

Approach:

• Literature Search: Identified 17 studies reporting significant modulation of NAD+ levels in adipose tissue.
• Gene Extraction: Extracted 113 unique NAD+-associated protein coding genes from the identified studies.
• Functional Bioinformatic Analyses: Conducted pathway enrichment analysis, protein-protein interaction analysis, and master regulator analysis.

Key Findings:

• Multiple canonical pathways significantly associated with NAD+-related genes include thermogenesis, PPAR signaling, AMPK signaling, and adipogenesis.
• Identified cellular pathways with currently lacking direct experimental evidence of NAD+ involvement, such as apelin and relaxin signaling pathways.
• Protein-protein interaction analysis revealed clusters related to cold-induced thermogenesis, inflammation, and cellular respiration.
• Master regulator analysis identified known regulators like leptin and PRDM16, as well as unexplored regulators in NAD+ function.

Interpretation:

Limitations:

• Notable lack of studies employing global transcriptomic approaches.
• Absence of multi-omics studies with coupled bioinformatic analyses.

Conclusion:

The study identifies potential targets for future experimental investigation regarding the role of NAD+ in adipose biology.