To evaluate the effects of immune checkpoint inhibitors on ovarian and endocrine function in a mouse model of triple negative breast cancer (TNBC).
Approach:
Mouse Model: Utilized a syngeneic TNBC mouse model to assess the impact of immunotherapies targeting PD-1, LAG-3, and TIM-3 on ovarian function.
Tumor-Free Model: Examined ovarian architecture, follicle abundance, estrus cyclicity, and ovulatory efficiency in a tumor-free mouse model following PD-1 blockade.
Key Findings:
Immunotherapies targeting PD-1, LAG-3, and TIM-3 did not affect the quality and abundance of ovarian follicles.
Estrus cyclicity and hormonal homeostasis remained unchanged in treated mice.
Ovarian architecture and ovulatory efficiency were unaffected by PD-1 blockade in tumor-free models.
Interpretation:
Limitations:
Findings are based on mouse models and may not directly translate to human physiology.
Long-term effects of immunotherapy on reproductive health were not assessed.
