To establish a cynomolgus macaque model for studying the pathogenesis of HAdV-55 and evaluating vaccines and therapeutics, addressing the gap in understanding this emerging respiratory pathogen.
Key Findings:
Infected macaques exhibited respiratory symptoms including nasal discharge, cough, weight loss, and increased respiratory and heart rates.
Lung imaging revealed peri-bronchial consolidation and ground-glass opacities.
Histopathology showed granulomatous inflammation and macrophage infiltration, resembling human disease.
Hematological analysis demonstrated early neutrophilia and basophilia, followed by eosinophilia and increased numbers of large unstained cells.
Cytokine profiling indicated early induction of IFN-γ, IFN-β, and IL-6, with delayed IL-8 elevation and IL-4 suppression.
Interpretation:
The cynomolgus macaque model effectively replicates key clinical and immunological features of HAdV-55 infection, providing a valuable platform for preclinical research.
Limitations:
The study primarily focuses on a single animal model, which may not fully represent all aspects of human disease, potentially limiting the generalizability of findings.
Further validation is needed to assess the model's utility in vaccine and therapeutic evaluations, particularly in diverse populations.
Conclusion:
This study establishes a cynomolgus macaque model that closely mimics HAdV-55-induced respiratory illness, facilitating future research on pathogenesis and treatment options.
by Sang Hwan Seo, Jung-ah Choi, Dae-Im Jung, Yunjeong Park, Eunji Yang, Chanmi Kim, Minkyung Ko, Seung Ho Baek, Jung Joo Hong, Soon-Hwan Kwon, Won-Tae Kim, Jun Young Lee, Manki Song
The 2025 US Department of Veterans Affairs and US Department of Defense asthma guideline introduces 21 evidence-based recommendations for primary care, redefining stepwise treatment, comorbidity management, and reliever strategy.
