Guanxinjing ameliorates coronary microvascular dysfunction in myocardial ischemia-reperfusion injury by alleviating inflammation and restoring endothelial function - Summary - MDSpire

Guanxinjing Enhances Coronary Microvascular Function in Myocardial Ischemia-Reperfusion Injury by Reducing Inflammation and Improving Endothelial Activity

  • By

  • Yaoting Wang

  • Lan Li

  • Liang Jin

  • Shikai Zhan

  • Hai Ren

  • Jiao Zhao

  • Qiuru Wang

  • Yiqiong Wang

  • Shudong Lin

  • Jiaxing Xin

  • Yao Dai

  • Yifan Zhou

  • Ting Zhang

  • Qian Liu

  • Yunyun Lu

  • Yiqi Zhu

  • Jiangyue Wu

  • Jiayan Lin

  • Shujing Zhang

  • Ling Zhang

  • July 17, 2026

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Objective:

To investigate the efficacy and mechanisms of Guanxinjing compound (GXJC) against myocardial ischemia-reperfusion injury (MIRI)-induced coronary microvascular dysfunction (CMD).

Approach:
  • Chemical Profiling: Performed using UHPLC-Q-Exactive HRMS to identify bioactive compounds in GXJC.
  • Network Pharmacology Analysis: Screened core targets and key pathways of GXJC, predicting the TNF signaling pathway as a potential mechanism.
  • In Vivo Studies: C57BL/6 mice received 7-day GXJC pretreatment before MIRI modeling, followed by evaluations using various staining and assessment techniques.
  • In Vitro Studies: Established a TNF-α-induced inflammatory injury model in HUVECs to dissect underlying mechanisms.
Key Findings:
  • Identified 256 bioactive compounds in GXJC.
  • GXJC treatment reduced myocardial infarct size and cardiomyocyte apoptosis.
  • Downregulated pro-inflammatory factors IL-6, IL-1β, and CCL2.
  • Modulated the eNOS/NO/ET-1 axis to alleviate endothelial dysfunction.
  • Lowered levels of FGL2 and p-selectin, reducing endothelial injury and thrombosis.
Interpretation:

Conclusion:

Original Source(s)

Guanxinjing ameliorates coronary microvascular dysfunction in myocardial ischemia-reperfusion injury by alleviating inflammation and restoring endothelial function

  • by Yaoting Wang, Lan Li, Liang Jin, Shikai Zhan, Hai Ren, Jiao Zhao, Qiuru Wang, Yiqiong Wang, Shudong Lin, Jiaxing Xin, Yao Dai, Yifan Zhou, Ting Zhang, Qian Liu, Yunyun Lu, Yiqi Zhu, Jiangyue Wu, Jiayan Lin, Shujing Zhang, Ling Zhang

  • July 17, 2026

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