Dysregulated lipid metabolites GML and GMO were associated with cytotoxic T cell function and serve as biomarkers for acute pulmonary embolism - Summary - MDSpire
Advertisement
Dysregulated lipid metabolites GML and GMO were associated with cytotoxic T cell function and serve as biomarkers for acute pulmonary embolism
To identify serum lipid biomarkers for acute pulmonary embolism (APE) and evaluate their diagnostic value, and to investigate the impact of glycerol monolaurate (GML) and glycerol monooleate (GMO) on cytotoxic T cell function.
Approach:
Study Design: A total of 436 subjects, including APE patients, healthy controls, and patients with related diseases, were enrolled.
Lipid Analysis: Serum samples were subjected to pseudotargeted lipidomics to screen differential lipid species, followed by targeted LC-MS/MS analysis to validate GML and GMO levels.
Cytotoxic T Cell Assessment: Flow cytometry was used to assess cytotoxic T cell markers such as granzyme B, perforin, and granulysin.
In Vitro Experiments: In vitro experiments examined the inhibitory effect of GML and GMO on Notch1 signaling and cytotoxic protein expression in T cells.
Diagnostic Performance Evaluation: ROC curve analyses were performed to evaluate the diagnostic performance of lipid biomarkers.
Key Findings:
A total of 203 upregulated and 57 downregulated serum lipids were identified in APE versus controls.
Serum GML and GMO levels were significantly elevated in APE compared to healthy controls and other diseases.
GML and GMO correlated with clinical risk stratification.
ROC analyses showed high sensitivity and specificity for GML and GMO individually.
Cytotoxic T cells from APE patients exhibited decreased granzyme B, perforin, and granulysin.
Interpretation:
Conclusion:
Elevated serum GML and GMO may act as biomarkers for APE.
