Modulation of chemokine secretion and oxidative stress in vitro in thyroid cells after exposure to thiocyanate, nitrate, or perchlorate - Summary - MDSpire
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Modulation of chemokine secretion and oxidative stress in vitro in thyroid cells after exposure to thiocyanate, nitrate, or perchlorate
To assess the effects of perchlorate, nitrate, and thiocyanate on cell viability, proliferation, reactive oxygen species (ROS) production, and chemokine expression in normal human thyroid cells and thyroid cancer cell lines, particularly focusing on their independent effects beyond iodide uptake inhibition.
Key Findings:
High-dose thiocyanate reduced viability in TPC-1 cells but not in NHT or 8505C cells, indicating cell-type specific responses.
Proliferative responses were modest and cell-type specific, mainly at the highest concentrations, suggesting a nuanced effect of the substances.
Perchlorate induced a biphasic increase in ROS production in NHT; thiocyanate slightly reduced ROS levels in TPC-1 cells, highlighting differential impacts on oxidative stress.
Thiocyanate consistently increased CXCL8 secretion in NHT and TPC-1 cells in a concentration-dependent manner, emphasizing its pro-inflammatory role.
CXCL10 protein was undetectable in all conditions despite modulation of CXCL10 mRNA expression, indicating a potential post-transcriptional regulation.
Interpretation:
Perchlorate, nitrate, and thiocyanate modulate oxidative stress and chemokine secretion in human thyroid cells, with thiocyanate promoting a pro-inflammatory phenotype that may have implications for thyroid health.
Limitations:
The study was conducted in vitro, which may not fully replicate in vivo conditions, and potential confounding factors were not addressed.
The effects observed may vary with different exposure durations and concentrations not tested, limiting the generalizability of the findings.
Conclusion:
The findings suggest that these thyroid disruptors may influence the thyroid tumor microenvironment and oxidative stress, potentially favoring tumor development, with implications for public health.
by Alessia Greco, Francesca Coperchini, Elena Franchi, Marco Denegri, Laura Croce, Marsida Teliti, Vincenzo Marotta, Flavia Magri, Giuseppina De Marco, Eleonora Ferrarini, Luisa Pignata, Patrizia Agretti, Massimo Tonacchera, Mario Rotondi
