Hyperacute Interleukin-1β Production and Neutrophil Extracellular Trap Formation in the Cerebral Circulation of Stroke Patients with Large Vessel Occlusion - Summary - MDSpire
Coming Soon: Introducing MDSpire News. Learn more
Conexiant’s news site is now MDSpire News. Learn more

Rapid Release of Interleukin-1β and Neutrophil Extracellular Trap Formation in the Cerebral Circulation of Patients with Large Vessel Occlusion Stroke

  • By

  • Justine Münsterberg

  • Caspar Brekenfeld

  • Hanna Englert

  • Marius Piepke

  • Regine J. Dress

  • Martin Oertel

  • Tina Fischer

  • Romy Hackbusch

  • Mingming Zha

  • Haodi Cai

  • Christian Casar

  • Ines S. Schädlich

  • Leo Winter

  • Alina Jander

  • Karoline Degenhardt

  • Maxim Bester

  • Fabian Flottmann

  • Uta Hanning

  • Brigitte Holst

  • Anna Worthman

  • Johanna Hiefner

  • Berenike Rutz

  • Eckhard Schlemm

  • Götz Thomalla

  • Bettina H. Clausen

  • Ann M. Stowe

  • Immo Prinz

  • Thiruma V. Arumugam

  • Markus Glatzel

  • Thomas Renné

  • Jens Fiehler

  • Eva Tolosa

  • Tim Magnus

  • Mathias Gelderblom

  • June 22, 2026

Share

Objective:

To investigate the hyperacute detrimental postischemic immune response in patients with large vessel occlusion (LVO) using a translational liquid biopsy approach.

Approach:
  • Study Design: Prospective enrollment of 54 patients with acute ischemic stroke caused by LVO of the middle cerebral artery within 24 hours after stroke onset, undergoing mechanical thrombectomy.
  • Sample Acquisition: Blood samples were collected from ischemic and nonischemic sites during mechanical thrombectomy to analyze immune responses.
  • Plasma Preparation: Whole blood was processed for plasma isolation and frozen for analysis.
  • Single-Cell RNA Sequencing: Peripheral blood mononuclear cells were isolated and sequenced to characterize immune cell responses.
  • Computational Analysis: Data analysis was performed using Seurat and SingleR for cell type annotation.
Key Findings:
  • Local production of IL-1β and NET formation are key hallmarks of the hyperacute intravascular immune reaction in stroke.
  • Immune cells from the intravascular space contribute to the postischemic immune response within the first few hours after stroke onset.
Interpretation:

The study provides insights into the hyperacute immune response in the human brain during LVO stroke.

Limitations:
  • The study is limited to a specific patient population undergoing mechanical thrombectomy.
  • Findings may not be generalizable to all ischemic stroke patients.
Conclusion:

The findings underscore the potential for targeted immunotherapies as an additive treatment to established recanalization procedures in patients with large infarct cores.

Original Source(s)

Hyperacute Interleukin-1β Production and Neutrophil Extracellular Trap Formation in the Cerebral Circulation of Stroke Patients with Large Vessel Occlusion

  • by Justine Münsterberg, Caspar Brekenfeld, Hanna Englert, Marius Piepke, Regine J. Dress, Martin Oertel, Tina Fischer, Romy Hackbusch, Mingming Zha, Haodi Cai, Christian Casar, Ines S. Schädlich, Leo Winter, Alina Jander, Karoline Degenhardt, Maxim Bester, Fabian Flottmann, Uta Hanning, Brigitte Holst, Anna Worthman, Johanna Hiefner, Berenike Rutz, Eckhard Schlemm, Götz Thomalla, Bettina H. Clausen, Ann M. Stowe, Immo Prinz, Thiruma V. Arumugam, Markus Glatzel, Thomas Renné, Jens Fiehler, Eva Tolosa, Tim Magnus, Mathias Gelderblom

  • June 22, 2026

Related Content

Multifactorial Nature of Childhood Stroke Presents a Major Challenge for Diagnosis and Management: A Population-Based Study From Southern Finland

Baptist Health Brain & Spine Care to Host 14th Annual Miami Neuroscience Symposium Nov. 4-6, Bringing Leading Experts Together to Explore Advances in Brain and Spine Care

Baptist Health will bring together leading physicians, researchers and healthcare professionals from South Florida and across the country for the 14th Annual Baptist Health Miami Neuroscience Symposium, taking place Nov. 4-6, 2026, at Hilton Miami Dadeland and virtually via Zoom.

  • by

  • August 21, 2026

  • 5 min
Pediatric Bowhunter Syndrome: What Do We Know?

Bowhunter syndrome (BHS) is a rare but important cause of posterior circulation stroke in children, resulting from vertebral artery compression during head rotation.

  • June 19, 2026

  • 1 min