Objective:

To investigate the effects of maternal high-fat diet (HFD) exposure on hypothalamic microglial development and TGFβ1 signaling in male offspring.

Approach:

Key Findings:

• Maternal HFD exposure reduced the developmental increase of TMEM119+ microglia in the mediobasal hypothalamus.
• HFD exposure was associated with altered microglial abundance and morphology across postnatal stages.
• Male offspring exhibited decreased pSMAD3 immunoreactivity and Parkin-associated signals in hypothalamic tissue.
• TGFβ1 supplementation increased pSMAD3 immunoreactivity and TMEM119-positive cell abundance in later juvenile stages.

Interpretation:

Limitations:

• The study primarily focuses on male offspring, limiting generalizability to females.
• The long-term effects of TGFβ1 supplementation on metabolic outcomes were not assessed.

Conclusion:

Attribution Notice

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