To evaluate the effectiveness of a multiplex PCR assay in identifying bloodstream pathogens and antimicrobial resistance genes in adult patients with bloodstream infections treated at an oncology hospital.
Key Findings:
The multiplex panel identified pathogens in 94% of positive blood cultures.
Results were available within 1 hour, compared to 24 to 48 hours for conventional methods.
The panel detected 96 microorganisms, while standard methods identified 92, with five discrepancies at the species level.
Gram-negative bacteria were predominant, especially Klebsiella pneumoniae and Escherichia coli.
The panel detected antimicrobial resistance genes in 59 bacteria, with a total of 107 genes identified.
Interpretation:
The FilmArray BCID2 panel provides rapid and accurate identification of bloodstream pathogens and antimicrobial resistance, facilitating earlier targeted therapy, which is crucial for improving outcomes in high-risk patients.
Limitations:
Inability to detect organisms not included in the assay.
Single-center design and relatively small sample size, which may limit generalizability.
Conclusion:
The FilmArray BCID2 panel correlates well with conventional methods and identifies antimicrobial resistance genes more quickly, enhancing treatment options for high-risk patients.
