Human seminal plasma suppresses neutrophil antimicrobial functions and promotes bacterial survival - Summary - MDSpire

Human seminal plasma suppresses neutrophil antimicrobial functions and promotes bacterial survival

  • By

  • Gabriel Mayoral-Andrade

  • Gabriela Vásquez-Martínez

  • Israel Cotzomi-Ortega

  • Gamaliel Sánchez-Orellana

  • Jeimy M. Lopez-Torres

  • Diana Zepeda-Orozco

  • Laura Pérez-Campos Mayoral

  • María Teresa Hernández-Huerta

  • Abraham Salvador Majluf-Cruz

  • María del Socorro Pina-Canseco

  • Margarito Martínez-Cruz

  • Gabriel Mayoral-Canseco

  • Carlos Alejandro Vásquez-Martínez

  • Víctor Cruz Hernández

  • Luz María Moreno Pombo

  • Adriana Carriedo Blanco

  • Eduardo Pérez-Campos Mayoral

  • Héctor Martínez-Ruiz

  • Eduardo Pérez-Campos

  • Juan de Dios Ruiz-Rosado

  • July 14, 2026

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Objective:

To investigate the immunomodulatory effects of human seminal plasma (HSP) on neutrophil antimicrobial functions and pathogen clearance.

Approach:
  • Experimental Design: Human neutrophils were stimulated with calcium ionophore A23187, phorbol 12-myristate 13-acetate (PMA), or uropathogenic Escherichia coli (UPEC) in the presence or absence of HSP. Neutrophils from NOX2- and PAD4-knockout mice were also utilized.
  • Assessment of Neutrophil Functions: Key neutrophil effector responses including reactive oxygen species (ROS) production, neutrophil extracellular trap (NET) formation, phagocytosis, and bacterial killing were quantified.
Key Findings:
  • HSP significantly suppressed ROS production and NET formation induced by A23187, PMA, and UPEC.
  • HSP reduced neutrophil phagocytic capacity and impaired bacterial killing.
  • The inhibition of NETosis by HSP was found to be PAD4-dependent but NOX2-independent.
  • Prostaglandin E2 levels in HSP were linked to its inhibitory effects on neutrophil functions.
Interpretation:

HSP acts as an inhibitor of neutrophil effector functions.

Limitations:
  • The study primarily focuses on in vitro experiments, which may not fully replicate in vivo conditions.
  • The specific contributions of other components in seminal plasma were not explored.
Conclusion:

HSP regulates neutrophil effector functions through distinct mechanisms, attenuating ROS production and NET formation, thereby impairing antibacterial activity.

Original Source(s)

Human seminal plasma suppresses neutrophil antimicrobial functions and promotes bacterial survival

  • by Gabriel Mayoral-Andrade, Gabriela Vásquez-Martínez, Israel Cotzomi-Ortega, Gamaliel Sánchez-Orellana, Jeimy M. Lopez-Torres, Diana Zepeda-Orozco, Laura Pérez-Campos Mayoral, María Teresa Hernández-Huerta, Abraham Salvador Majluf-Cruz, María del Socorro Pina-Canseco, Margarito Martínez-Cruz, Gabriel Mayoral-Canseco, Carlos Alejandro Vásquez-Martínez, Víctor Cruz Hernández, Luz María Moreno Pombo, Adriana Carriedo Blanco, Eduardo Pérez-Campos Mayoral, Héctor Martínez-Ruiz, Eduardo Pérez-Campos, Juan de Dios Ruiz-Rosado

  • July 14, 2026

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