Objective:

To investigate CELA2A expression in gastrointestinal tissues and circulation in relation to obesity and Roux-en-Y gastric bypass (RYGB), highlighting its potential role in metabolic signaling.

Key Findings:

• CELA2A expression increased in gastric mucosa after RYGB but decreased in the jejunum, indicating tissue-specific responses.
• Circulating CELA2A levels decreased post-RYGB, suggesting altered metabolic signaling.
• Before surgery, jejunal CELA2A levels were positively associated with HbA1c, indicating a potential link to glycemic control.
• In high-fat diet-fed mice, jejunal CELA2A expression increased while gastric expression decreased, reflecting dietary influences.

Interpretation:

CELA2A exhibits tissue-specific regulation in obesity and following bariatric surgery, suggesting its potential role in gastrointestinal metabolic signaling, possibly through modulation of gut hormones.

Limitations:

• Detailed cellular localization of CELA2A in human tissue was not established, limiting understanding of its functional role.
• Circulating and tissue levels of CELA2A were not directly correlated, raising questions about their relationship.

Conclusion:

Further studies are required to define the cellular origin and functional role of CELA2A in glucose homeostasis, emphasizing its potential as a therapeutic target.