To investigate the molecular mechanisms of Huoxue Jiegu Compound Capsule (HXJGCC) in promoting tibial fracture healing, with a specific focus on angiogenesis and its underlying pathways.
Key Findings:
Identified 209 candidate active compounds and 185 overlapping targets related to HXJGCC, with key hub targets including AKT1, STAT3, IL6, BCL2, EGFR, and JUN, which play significant roles in angiogenesis-related pathways.
In vivo experiments showed HXJGCC significantly increased vascular density and improved vascular architecture in the fracture region.
qRT-PCR confirmed upregulation of angiogenesis-associated genes (AKT1, STAT3, IL6, EGFR), indicating activation of pro-angiogenic regulatory networks.
Interpretation:
HXJGCC enhances tibial fracture healing by promoting angiogenesis and improving the microvascular environment, potentially through HIF-1/PI3K–Akt signaling pathways, which coordinate inflammatory responses and vascular remodeling.
Limitations:
The study primarily focused on a rabbit model, which may limit the generalizability of findings to humans.
Further research is needed to fully elucidate the multi-target mechanisms of HXJGCC, including human clinical trials.
Conclusion:
HXJGCC accelerates fracture healing through angiogenesis, suggesting its potential as a therapeutic strategy for bone regeneration.
