Development of a BM7G(TKO/hCD46/hCD55/hTHBD/hEPCR) donor pig with endogenous promoter-driven transgenes for xenotransplantation - Summary - MDSpire

Development of a BM7G(TKO/hCD46/hCD55/hTHBD/hEPCR) donor pig with endogenous promoter-driven transgenes for xenotransplantation

  • By

  • Cong Xia

  • Meng Lian

  • Bingxiu Ma

  • Hongliang Yu

  • Renquan Zhang

  • Lijia Wen

  • Xueliang Wang

  • Yu Zhao

  • Zhen Ouyang

  • Yinghua Ye

  • Xiner Feng

  • Han Wu

  • Liangxue Lai

  • June 23, 2026

  • 0 min

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Objective:

To develop a genetically modified pig model with stable multi-gene expression for xenotransplantation.

Approach:
    Key Findings:
    • Complete absence of the three glycan antigens at cellular and tissue levels in BM7G genetically modified pigs.
    • Stable expression of four protective proteins in vascular endothelial cells and major organs.
    • hCD55 and hCD46 were widely expressed, while hTHBD and hEPCR were specifically expressed in vascular regions.
    • In-vitro assays showed reduced binding of human antibodies and decreased complement-dependent cytotoxicity.
    Interpretation:

    The combination of xenoantigen knockout and endogenous promoter-driven expression of protective genes resulted in a genetically modified pig model.

    Conclusion:

    The BM7G genetically modified pig model provides a resource for preclinical research in xenotransplantation.

Original Source(s)

Development of a BM7G(TKO/hCD46/hCD55/hTHBD/hEPCR) donor pig with endogenous promoter-driven transgenes for xenotransplantation

  • by Cong Xia, Meng Lian, Bingxiu Ma, Hongliang Yu, Renquan Zhang, Lijia Wen, Xueliang Wang, Yu Zhao, Zhen Ouyang, Yinghua Ye, Xiner Feng, Han Wu, Liangxue Lai

  • June 23, 2026

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