An advanced, highly sensitive and versatile Cypridina luciferase-based HPV pseudovirus system for broad-spectrum neutralization and non-invasive In vivo monitoring - Summary - MDSpire

An advanced, highly sensitive and versatile Cypridina luciferase-based HPV pseudovirus system for broad-spectrum neutralization and non-invasive In vivo monitoring

  • By

  • Hyemi Kim

  • Chai Won Kim

  • Kihyuck Kwak

  • May 29, 2026

  • 0 min

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Objective:

To develop a pseudovirus platform using Cypridina luciferase for high-throughput neutralization assays of HPV vaccines.

Key Findings:
  • cLuc-PsVs showed robust infectivity and excellent analytical linearity.
  • The system eliminated the need for serum thermal inactivation while maintaining a high correlation with traditional SEAP assays.
  • The platform enabled non-invasive tracking of mucosal infection across all 28 HPV genotypes.
Interpretation:

Limitations:
  • The study does not address potential limitations in the generalizability of the findings across all HPV genotypes.
  • Further validation in diverse clinical settings is necessary to confirm the platform's effectiveness.
Conclusion:

The cLuc-PsV platform is a tool for preclinical screening of multivalent HPV vaccine candidates.

Original Source(s)

An advanced, highly sensitive and versatile Cypridina luciferase-based HPV pseudovirus system for broad-spectrum neutralization and non-invasive In vivo monitoring

  • by Hyemi Kim, Chai Won Kim, Kihyuck Kwak

  • May 29, 2026

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