Dynamic urinary proteomics integrates single-cell and spatial transcriptomics to reveal tumour microenvironment and predict immunotherapy response in biliary tract cancer - Summary - MDSpire

Dynamic urinary proteomics integrates single-cell and spatial transcriptomics to reveal tumour microenvironment and predict immunotherapy response in biliary tract cancer

  • By

  • Shanshan Wang

  • Zhengguang Guo

  • Boyu Sun

  • Kai Liu

  • Jiashuo Chao

  • Ziyu Xun

  • Yunchao Wang

  • Zibo Xu

  • Ziyue Huang

  • Hao Wang

  • Yang Tan

  • Nan Zhang

  • Mingjian Piao

  • Longhao Zhang

  • Chengjie Li

  • Shuofeng Li

  • Jiongyuan Li

  • Haidan Sun

  • Feng Qi

  • Aiwei Wang

  • XiaoBo Yang

  • Chengpei Zhu

  • Hanping Wang

  • Wei Sun

  • Haitao Zhao

  • July 1, 2026

  • 0 min

Share

Objective:

To investigate urinary proteomics as a non-invasive method for predicting immune checkpoint inhibitor (ICI) responsiveness and understanding tumor microenvironment dynamics in biliary tract cancer (BTC), addressing the critical need for reliable biomarkers.

Key Findings:
  • Urinary proteomics provides a non-invasive approach to predict ICI responsiveness and capture tumor dynamics, potentially transforming patient management.
  • Protein tyrosine phosphatase non-receptor 13 is identified as a promising biomarker and potential therapeutic target, highlighting its relevance in enhancing ICI outcomes.
  • The study positions urinary proteomics as a valuable tool for early prediction and monitoring of ICI responsiveness in BTC, paving the way for future research.
Interpretation:

The findings suggest that urinary proteomics can serve as a dynamic biomarker for monitoring immune responses and tumor microenvironment changes in BTC patients undergoing immunotherapy, potentially improving patient outcomes.

Limitations:
  • The study is preliminary and requires further validation in larger cohorts to confirm findings.
  • Potential biases in sample collection and analysis, such as selection bias and variability in urine sample handling, may affect the generalizability of the findings.
Conclusion:

Urinary proteomics may enhance the understanding of ICI responsiveness and tumor dynamics in biliary tract cancer, supporting its role as a non-invasive biomarker with significant clinical implications.

Original Source(s)

Dynamic urinary proteomics integrates single-cell and spatial transcriptomics to reveal tumour microenvironment and predict immunotherapy response in biliary tract cancer

  • by Shanshan Wang, Zhengguang Guo, Boyu Sun, Kai Liu, Jiashuo Chao, Ziyu Xun, Yunchao Wang, Zibo Xu, Ziyue Huang, Hao Wang, Yang Tan, Nan Zhang, Mingjian Piao, Longhao Zhang, Chengjie Li, Shuofeng Li, Jiongyuan Li, Haidan Sun, Feng Qi, Aiwei Wang, XiaoBo Yang, Chengpei Zhu, Hanping Wang, Wei Sun, Haitao Zhao

  • July 1, 2026

Related Content

Bone health in neuroendocrine tumors and prognostic implications beyond skeletal metastases

The role of protein lactylation in skin diseases: from molecular mechanisms to potential therapeutics

Development and validation of a prediction model for psychological distress in patients with differentiated thyroid cancer undergoing ¹³¹I therapy