Quercetagetin-rich flavonoids from marigold induce apoptosis inhibit metastasis of non-small cell lung cancer via the p53/p21 signaling pathway - Summary - MDSpire
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Quercetagetin-rich flavonoids from marigold induce apoptosis inhibit metastasis of non-small cell lung cancer via the p53/p21 signaling pathway
To investigate the in vitro and in vivo inhibitory effects of quercetagetin-rich marigold flavonoids (QG-MF) on non-small cell lung cancer (NSCLC).
Approach:
In vitro experiments: Conducted on A549 and H661 NSCLC cell lines to assess cell proliferation, migration, invasion, apoptosis, and cycle distribution.
In vivo experiments: Evaluated the anti-tumor efficacy of QG-MF using A549 NSCLC xenograft mouse models.
Key Findings:
QG-MF significantly suppressed proliferation, migration, and invasion of A549 and H661 NSCLC cells.
Induced apoptosis and cell cycle arrest in a concentration-dependent manner.
Upregulated p53, p21, Bax, and caspase-3, while downregulating Bcl-2 in A549 and H661 NSCLC cells.
siRNA-mediated p53 silencing abrogated the anti-proliferative and pro-apoptotic effects of QG-MF.
In vivo, QG-MF markedly inhibited growth of A549 xenograft tumors without systemic toxicity.
Interpretation:
QG-MF exerts anti-NSCLC activity by inducing apoptosis and inhibiting proliferation/metastasis via the p53/p21 signaling pathway.
Conclusion:
QG-MF may serve as a natural candidate for the development of novel anti-NSCLC agents.
