Potential neuroprotective and anticonvulsant effects of Ganoderma lucidum ethanol extract and its impact on affective comorbidities associated with pentylenetetrazol kindling model of epilepsy: behavioral, biochemical and histological studies - Summary - MDSpire
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Potential neuroprotective and anticonvulsant effects of Ganoderma lucidum ethanol extract and its impact on affective comorbidities associated with pentylenetetrazol kindling model of epilepsy: behavioral, biochemical and histological studies
To evaluate the neuroprotective and anticonvulsant effects of Ganoderma lucidum extract (GLE) in a pentylenetetrazol (PTZ) kindling model of epilepsy and its impact on affective comorbidities.
Approach:
Study Design: Four groups of male Wistar rats were assigned: control, PTZ, GLE-300, and Diazepam. The PTZ model was induced with intraperitoneal doses of PTZ every 48 hours for 21 days.
Behavioral Assessment: Seizure severity was assessed using the Racine five-point score. Anxious and depressive behaviors were evaluated through the open field test, elevated plus maze test, and forced swim test.
Biochemical Analysis: Markers of oxidative stress (MDA, NO) and antioxidant enzyme activities (SOD, catalase) were measured using various biochemical methods.
Histological Study: Nissl staining was used to quantify neuronal loss in the prefrontal cortex.
Key Findings:
GLE significantly reduced the severity of convulsive seizures (p < 0.001).
