Objective:

To construct a comprehensive, label-free molecular atlas of the Alzheimer’s brain and identify region-specific chemical changes.

Key Findings:

• Significant regional heterogeneity in Alzheimer’s-related molecular changes was observed.
• Memory-associated areas like the hippocampus and cortex showed stronger deviations from healthy tissue.
• Metabolic differences, including variations in cholesterol and glycogen, were identified across multiple brain regions.

Interpretation:

The findings suggest that Alzheimer’s disease involves broader disruptions in brain structure and energy balance, beyond just protein aggregation.

Limitations:

• The study was conducted in an animal model, which may not fully replicate human Alzheimer's pathology.
• Further research is needed to extend findings to other disease models or earlier stages of Alzheimer's.

Conclusion:

The framework provides a more comprehensive view of Alzheimer’s-associated molecular changes, potentially aiding in understanding disease progression.