Label-Free Multiphoton Microscopy for intraoperative Identification of Glioma Features and Tumor Heterogeneity - Summary - MDSpire

Label-Free Multiphoton Microscopy for intraoperative Identification of Glioma Features and Tumor Heterogeneity

  • By

  • Duncker, León V.

  • Richter, Sven

  • Galli, Roberta

  • Meinhardt, Matthias

  • Hoffmann, Leon C.

  • Kirsche, Katrin

  • Großer, Lidia

  • Temme, Achim

  • Eyüpoglu, Ilker Y.

  • Uckermann, Ortrud

  • June 22, 2026

  • 0 min

Share

Objective:

To investigate the use of label-free multiphoton imaging modalities for intraoperative glioma grading.

Approach:
    Key Findings:
    • CARS detected lipid droplets, AF showed cellular structures, and SHG visualized blood vessels and extracellular matrix remodeling.
    • Statistically significant differences in feature prevalence were found among tumor types, particularly between WHO grade 2 and grade 4 gliomas.
    • Integration of imaging features into a numerical score indicated malignancy, with the highest scores in WHO grade 4 tumors.
    Interpretation:

    Label-free multiphoton imaging has potential as an intraoperative diagnostic tool for glioma malignancy assessment.

    Limitations:
    • Substantial variability across patients and within individual tissue samples.
    Conclusion:

    Label-free multiphoton imaging can provide enhanced real-time information on glioma malignancy during surgery.

Original Source(s)

Label-Free Multiphoton Microscopy for intraoperative Identification of Glioma Features and Tumor Heterogeneity

  • by Duncker, León V., Richter, Sven , Galli, Roberta , Meinhardt, Matthias , Hoffmann, Leon C., Kirsche, Katrin , Großer, Lidia , Temme, Achim , Eyüpoglu, Ilker Y., Uckermann, Ortrud

  • June 22, 2026

Related Content

Identification of an NFIA::CBFA2T3 fusion in cerebrospinal fluid confirms the diagnosis of pediatric CNS myeloid sarcoma with erythroid differentiation: a case report and literature review

Intraoperative radiotherapy after resection of newly diagnosed brain metastases in adult patients - results of a prospective phase II trial (INTRAMET)

Peripheral blood monocytes could be associated with brain metastasis and affect patient prognosis in breast cancer: a retrospective study