To evaluate the performance of capillary electrophoresis-mass spectrometry (CE-MS) across various laboratories for its potential use in top-down proteomics.
Approach:
Study Design: An international benchmarking study was conducted across 12 laboratories to evaluate CE-MS performance using diverse setups and user experiences.
Sample Selection: Commercially available protein mixes and intact protein cell lysates were used to minimize variability and enhance reproducibility.
Communication Strategy: Regular update meetings were held to ensure project alignment and address issues promptly.
Key Findings:
CE-MS shows exceptional separation efficiency and low sample consumption.
Many barriers to CE-MS adoption have been reduced, including improved interfaces and stability.
CE-MS can complement established LC-MS approaches in top-down proteomics.
Interpretation:
The study suggests that CE-MS has made significant progress in robustness and reproducibility, which may facilitate its broader use in top-down proteomics.
Limitations:
The findings are based on specific CE-MS setups, which may not be applicable to all laboratory environments.
Differences in user experience and laboratory conditions may influence the reproducibility of results.
Conclusion:
The benchmarking study serves as a reference for future CE-MS applications, promoting standardization and potential adoption in proteoform analysis.
