Objective:

To discuss the emerging role of long-read sequencing (LRS) in cancer liquid biopsy, summarizing recent studies across multiple cancer types.

Approach:

Key Findings:

• LRS can detect complex genomic rearrangements and epigenetic modifications that SRS cannot.
• Studies have identified novel structural variants and gene fusions using LRS in various cancer types.
• LRS supports direct methylation profiling without bisulfite conversion, preserving important genomic features.

Interpretation:

Limitations:

• Pre-analytical variability in sample collection and processing.
• High costs associated with LRS technologies.
• Increased computational demands for data analysis.

Conclusion:

Attribution Notice

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