Single-cell sequencing uncovers clonal dynamics profiles and therapeutic resistance biomarkers in relapsed and refractory peripheral T-cell lymphoma - Summary - MDSpire

Single-cell sequencing uncovers clonal dynamics profiles and therapeutic resistance biomarkers in relapsed and refractory peripheral T-cell lymphoma

  • By

  • Ning Zhang

  • Jingwei Yu

  • Hengqi Liu

  • Zechao Hu

  • Shen Meng

  • Cong Sun

  • Yaxiao Lu

  • Xia Liu

  • Zheng Song

  • Lanfang Li

  • Lihua Qiu

  • Zhengzi Qian

  • Bin Meng

  • Wenchen Gong

  • Shiyong Zhou

  • Xianhuo Wang

  • Huilai Zhang

  • July 10, 2026

  • 0 min

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Objective:

To characterize the transcriptional profiles of malignant T-cell clones and reactive T lymphocytes in relapsed and refractory peripheral T-cell lymphoma (R/R PTCL) and identify potential biomarkers associated with treatment resistance.

Approach:
  • Methodology: Single-cell RNA sequencing and single-cell T-cell receptor sequencing were performed on seven tumor samples from six patients with R/R PTCL.
Key Findings:
  • Significant upregulation of genes related to cell proliferation, oncogenic signaling, and immune modulation in R/R PTCL.
  • Identification of protumorigenic ligand-receptor interactions (e.g., CXCL13–CXCR5, CCL5–CCR5) that may facilitate immune evasion.
Interpretation:

The transcriptional heterogeneity of malignant T-cell clones in R/R PTCL may contribute to resistance to epigenetic therapies.

Limitations:
  • The study involved a small sample size of six patients.
  • Findings may not be generalizable to all subtypes of PTCL.
Conclusion:

This study highlights the complexity of treatment resistance in R/R PTCL.

Original Source(s)

Single-cell sequencing uncovers clonal dynamics profiles and therapeutic resistance biomarkers in relapsed and refractory peripheral T-cell lymphoma

  • by Ning Zhang, Jingwei Yu, Hengqi Liu, Zechao Hu, Shen Meng, Cong Sun, Yaxiao Lu, Xia Liu, Zheng Song, Lanfang Li, Lihua Qiu, Zhengzi Qian, Bin Meng, Wenchen Gong, Shiyong Zhou, Xianhuo Wang, Huilai Zhang

  • July 10, 2026

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