To explore the DNA methylation changes in lung tissue of mice with allergic inflammation induced by Blomia tropicalis allergens compared to saline-exposed controls.
Approach:
Sensitization and Challenge: BALB/c mice were sensitized and challenged with B. tropicalis extract or purified allergens Blo t 2 and Blo t 13, or saline solution.
DNA Methylation Assessment: Lung DNA methylation was assessed across over 285,000 CpG sites using the Infinium Mouse Methylation BeadChip.
Key Findings:
Allergen-exposed mice exhibited distinct lung DNA methylation profiles compared to controls.
137, 179, and 313 differentially methylated regions (DMRs) were identified in mice exposed to Blo t 2, Blo t 13, and B. tropicalis extract, respectively.
Key genes in DMRs included protocadherin alpha genes, cadherin 8, and interleukin 11 receptor alpha.
Differentially methylated genes were enriched in biological processes related to cell adhesion and nervous system development.
Significant differences in mRNA levels of neuronal growth genes and proapoptotic gene Bcl2l11 were observed.
Interpretation:
Exposure to B. tropicalis allergens is linked to lung DNA methylation changes in genes related to allergic asthma and inflammation.
Limitations:
The study is exploratory and hypothesis-generating, requiring further validation.
Findings are based on a mouse model, which may not fully replicate human responses.
Conclusion:
B. tropicalis exposure alters DNA methylation in lung tissues.
by Kevin Llinás-Caballero, Nathalie Acevedo, Simon Kebede Merid, Karen Donado, Hector Espinoza, Ernesto Mondol, Randy Reina, Ronald Regino, Inés Benedetti, Josefina Zakzuk, Leonardo Puerta, Erik Melén, Luis Caraballo
A structured overview of recent FDA recalls, corrections, and alerts involving medications, ventilators, insulin delivery systems, cardiovascular devices, anesthesia products, and other equipment used in clinical practice.
