Objective:

To investigate whether casein enzymatic hydrolysate (CEH) protects against Salmonella Typhimurium-induced intestinal injury and to elucidate the underlying mechanisms.

Approach:

Key Findings:

• CEH improved survival and reduced clinical disease severity.
• CEH preserved mucosal architecture and restored barrier proteins.
• CEH promoted intestinal stem cell regeneration and reshaped gut microbiota.
• L-lactate levels increased, enhancing Paneth cell function and Wnt3A expression.
• Blockade of GPR81 or Wnt negated CEH and lactate benefits.

Interpretation:

CEH alleviates S. Typhimurium-induced enteritis through a microbiota-lactate-GPR81-Wnt3A-ISC axis.

Limitations:

• Study conducted in a mouse model, which may not fully replicate human responses.
• Long-term effects of CEH and lactate supplementation were not assessed.

Conclusion:

CEH is a promising nutritional strategy to mitigate infection-induced intestinal injury and promote regeneration in adult hosts.

Attribution Notice

This content is an AI-generated, fully rewritten summary based on a published scholarly article. It does not reproduce the original text and is not a substitute for the original publication. Readers are encouraged to consult the source for full context, data, and methodology.