To review the application of Raman spectroscopy and its enhanced variants in detecting pandemic-prone viruses, emphasizing its significance in pandemic preparedness.
Key Findings:
Raman-based methods can rapidly detect influenza and identify SARS-CoV-2 with high accuracy.
Multiplexed detection of viruses in complex samples is achievable.
Research focus is skewed towards accessible viruses, neglecting high-risk pathogens like Lassa and Marburg.
Interpretation:
While Raman spectroscopy shows promise in virology, significant technical challenges such as weak signal intensity and regulatory hurdles hinder its clinical application.
Limitations:
Weak signal intensity and fluorescence interference limit detection capabilities.
Lack of standardization affects reproducibility.
Biosafety constraints restrict research on high-risk pathogens, impacting overall research focus.
Conclusion:
Raman spectroscopy has potential for pandemic preparedness but requires overcoming technical barriers and regulatory challenges to be effective in real-world diagnostics.
