To characterize serum exosomal miRNA profiles and construct a robust diagnostic model to distinguish treatment-naïve CPP patients from healthy controls.
Key Findings:
92 differentially expressed small RNAs identified, predominantly miRNAs.
Four candidate miRNAs (hsa-miR-6747-3p, hsa-miR-6873-5p, hsa-miR-615-3p, hsa-miR-6886-3p) validated.
Diagnostic signature achieved an AUC of 0.912 with 91.3% sensitivity and 88.9% specificity.
Interpretation:
The four-miRNA signature provides a highly sensitive and specific non-invasive diagnostic tool for CPP, linking circulating markers to the GnRH and metabolic pathways.
Limitations:
Study limited to a single cohort and may require validation in diverse populations.
Potential inter-individual variability in miRNA expression not fully addressed.
Conclusion:
This study establishes a novel serum exosomal four-miRNA signature as a promising alternative to invasive testing for diagnosing CPP, paving the way for precision diagnostics in pediatric endocrinology.
