To investigate the intrinsic mechanism by which electroacupuncture (EA) treats embryo implantation dysfunction in mice from the perspective of exosomal miRNAs.
Key Findings:
Co-culture with Ishikawa cells significantly promoted the proliferation and invasion of HTR-8/Svneo cells.
Exosomes from both Ishikawa and HEC-1-A cells enhanced HTR-8/Svneo cell proliferation and invasion.
miR-30c-5p was expressed at low levels in Ishikawa-derived exosomes and high levels in HEC-1-A-derived exosomes.
EA reduced miR-30c-5p expression in the endometrium, increased implantation sites, and alleviated endometrial injury.
Inhibition of miR-30c-5p in HTR-8/Svneo cells promoted cell proliferation and invasion and increased phosphorylation of FAK and JNK.
Interpretation:
EA may ameliorate embryo implantation dysfunction in mice by downregulating miR-30c-5p in the endometrium.
Limitations:
Conclusion:
EA may improve embryo implantation dysfunction through the modulation of miR-30c-5p.
