Frozen melanoma tissues yield extracellular vesicles with preserved diagnostic and immunogenic properties - Summary - MDSpire

Frozen melanoma tissues yield extracellular vesicles with preserved diagnostic and immunogenic properties

  • By

  • Daniele D´Arrigo

  • Kyong-Su Park

  • Cecilia Lässer

  • Georgios Panagiotis Sigalas

  • Emma Symonds

  • Ornella Urzí

  • Camilla Locatelli

  • Roger Olofsson Bagge

  • Jan Lötvall

  • Rossella Crescitelli

  • May 21, 2026

  • 0 min

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Objective:

To determine whether EVs isolated from fresh and frozen human melanoma tissues are comparable in terms of protein cargo and immunotherapeutic properties.

Approach:
    Key Findings:
    • EVs derived from frozen melanoma tissues retain key characteristics similar to those from fresh tissues.
    • Cryopreserved tissues can serve as a reliable source of EVs for cancer diagnostics and immunotherapy development.
    Interpretation:

    The study supports the feasibility of using cryopreserved melanoma tissues for EV isolation without significant loss of diagnostic and immunogenic properties.

    Limitations:
    • Limited number of patient samples (N = 9).
    • Potential artifacts introduced during the freezing process.
    Conclusion:

    Cryopreserved melanoma samples are a viable source for isolating EVs with preserved characteristics relevant for cancer diagnostics and immunotherapy.

Original Source(s)

Frozen melanoma tissues yield extracellular vesicles with preserved diagnostic and immunogenic properties

  • by Daniele D´Arrigo, Kyong-Su Park, Cecilia Lässer, Georgios Panagiotis Sigalas, Emma Symonds, Ornella Urzí, Camilla Locatelli, Roger Olofsson Bagge, Jan Lötvall, Rossella Crescitelli

  • May 21, 2026

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