To demonstrate the effectiveness of next-generation metagenomic sequencing (mNGS) in diagnosing complex infections in an immunocompromised patient case.
Key Findings:
Histopathological examination revealed granulomatous inflammation with alcohol-resistant bacilli and an undiagnosed cutaneous T-cell lymphoma.
Conventional tests identified multiple pathogens but lacked clarity due to their heterogeneous distribution.
mNGS provided crucial diagnostic insights that conventional methods could not achieve, highlighting its potential in managing complex infections in immunocompromised patients, especially given the limitations of traditional diagnostics.
Limitations:
Results from mNGS were available too late to influence treatment.
Environmental contamination and human DNA presence can complicate result interpretation, potentially leading to false positives.
Conclusion:
Early application of mNGS could significantly improve diagnostic precision and therapeutic decisions in critically ill immunocompromised patients.
by Rossana Scutari, Valeria Fox, Martina Mastropaolo, Vanessa Fini, Marco Mussa, Paolo Bigliano, Luna Colagrossi, Gianluca Vrenna, Andrea Perinzano, Silvia Scabini, Carlo Federico Perno, Andrea Calcagno
