To characterize immune cell composition, transcriptional programs, and intercellular communication associated with PRRSV infection in bronchial lymph nodes.
Approach:
Method: label
Method: text
Key Findings:
HP-PRRSV HV infection reduced the proportions of multiple T-cell subsets, including naive T cells, proliferating T cells, and central memory T cells (Tcm), while increasing B cell proportions in BLNs.
Infection disrupted T-cell egress, leading to cytotoxic T lymphocyte accumulation in BLNs.
Impaired crosstalk between T follicular helper cells and germinal center B cells was observed, potentially affecting neutralizing antibody production.
A novel RAG1+ CD4+CD8+ double-positive T-cell subset was identified.
Interpretation:
The findings suggest a multifaceted understanding of PRRSV immune evasion mechanisms.
Limitations:
The study primarily focuses on the immune landscape in bronchial lymph nodes and may not fully represent systemic immune responses, limiting the generalizability of the findings.
Conclusion:
The study provides insights into the mechanisms underlying PRRSV-mediated adaptive immunosuppression, which may inform vaccine design and antiviral strategies.
