Objective:

To test the hypothesis that different exercise modalities (aerobic, resistance, combined) differentially remodel gut microbiota and serum metabolic profiles, and improve skeletal muscle function and morphology through the gut–muscle axis in high-fat diet-induced obese mice.

Approach:

Key Findings:

• All exercise groups showed improved treadmill endurance and grip strength compared to the high-fat diet group, along with reduced lipid accumulation and fibrosis.
• The combined exercise group exhibited the most significant improvements in muscle fiber morphology, increased slow-twitch fibers, and elevated mitochondrial DNA content.
• Gut microbiota analysis revealed reduced α-diversity in the high-fat diet group, with the combined exercise group restoring diversity and specific microbial changes identified.
• Distinct microbial changes were identified across exercise modalities, including specific genera enrichment such as Blautia and Romboutsia.
• Metabolomic analysis identified 278 differential metabolites with modality-specific regulatory patterns.

Interpretation:

Aerobic, resistance, and combined exercise modalities differentially improved skeletal muscle dysfunction in obese mice, linked to changes in gut microbiota and metabolic profiles.

Conclusion:

The study demonstrates that exercise modality influences skeletal muscle performance and gut microbiota composition in high-fat diet-induced obesity, with specific associations to metabolic profiles.

Attribution Notice

This content is an AI-generated, fully rewritten summary based on a published scholarly article. It does not reproduce the original text and is not a substitute for the original publication. Readers are encouraged to consult the source for full context, data, and methodology.