To profile dynamic phosphorylation events in melanoma cells following CCL5 stimulation and to dissect CCR5-mediated phosphorylation distinct from other CCL5 receptors.
Approach:
Key Findings:
CCL5 treatment modulated 256, 134, and 83 phosphosites at 5, 10, and 30 min respectively.
A total of 393 phosphosites across 315 phosphoproteins exhibited significant regulation.
52 phosphoproteins were identified with distinct temporal dynamics in CCR5 knockout versus wild type cells.
Gene Ontology analysis indicated involvement in various biological processes, particularly cell cycle regulation.
Phosphorylation activation of CEP131, KHDRBS1, and MAPK6 was observed upon CCL5 stimulation, which was prevented by CCR5 knockout.
Interpretation:
The findings provide a comprehensive phosphorylation resource for understanding the molecular mechanisms of CCL5/CCR5 in tumor progression.
Conclusion:
The study enhances the understanding of CCL5/CCR5 signaling in melanoma and identifies key phosphorylation events.
