White matter microdissection of the lateral aspect of the brain: 2-dimensional video demonstration

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The white matter dissection technique was popularized by Josef Klingler, who developed a fixation protocol enhancing dissection clarity.
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Klingler's method remains the reference standard, although refinements like intra-carotid formalin perfusion have been introduced.
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Neurosurgeons can improve their understanding of brain anatomy through white matter bundle topography, aiding surgical strategy.
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The video demonstration provides a step-by-step guide for lateral brain dissection, addressing the lack of detailed visual resources.
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The Klingler fixation protocol involves fixation, freezing, and thawing phases, crucial for successful white matter dissection.

Acta Neurochirurgica

by Luca Zanuttini, Victor E. Staartjes, Shao-Ching Chen, Chun-Fu Lin, Sanford P. C. Hsu, Carolina Martins, Hung Tzu Wen, Carlo Serra, Uğur Türe
August 7, 2025

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