Pooled CRISPR Screening Identifies Genes Essential for Erythroblast Enucleation

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Erythroblast enucleation involves chromatin condensation and cell cycle exit, organelle clearance, nuclear polarization, and nuclear extrusion.
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The molecular mechanisms driving erythroblast enucleation remain incompletely understood, in part because existing erythroid cell lines enucleate infrequently.
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The authors developed a pooled CRISPR-Cas9 screening strategy using enucleated red cells derived from primary human HSPCs.
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The screen used sgRNA abundance measured by sequencing to compare unsorted cells with FACS-sorted enucleated cells.
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Primary human CD34+ HSPCs were differentiated ex vivo, transduced with a pooled sgRNA library, and analyzed for gene-level enrichment.

American Journal Of Hematology

by Marilou Tetard, Tianjian Lin, Nana A. Peterson, Rebekah C. Gullberg, Yann Le Guen, Jasmine Moshiri, John G. Doench, Elizabeth S. Egan
October 6, 2026

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