Refining Single-Cell Metabolomics with Ion Mobility

Ion mobility-resolved mass cytometry expands metabolite coverage across thousands of single cells

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A mass spectrometry workflow integrates ion mobility separation with mass cytometry for high-throughput single-cell metabolomics.
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The method enhances metabolite coverage by addressing low sensitivity and high variability in single-cell metabolomics.
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Ion mobility-enabled selective ion accumulation enriches low-mass metabolites and reduces interference from abundant species.
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The researchers developed MetCell, a computational workflow for peak detection and metabolite annotation in single-cell datasets.
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The approach revealed metabolic variations in liver cells from young and aged mice, highlighting its potential for studying cellular metabolism.

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