Evaluating the Diagnostic Precision of Rapid Tests for Vibrio cholerae Serogroups O1 and O139: A Systematic Review and Meta-Analysis

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Cholera, an acute secretory diarrheal disease caused by *Vibrio cholerae*, accounted for 804 721 reported cases and 5805 deaths across 33 countries in 2024.
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Among more than 200 identified serogroups, *V cholerae* O1 and O139 are the only serogroups that cause epidemics; timely, accurate detection is critical for targeted public health responses.
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Conventional fecal culture requires biochemical and serologic confirmation and takes several days, limiting its usefulness for rapid decision-making during outbreaks.
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Previous systematic reviews and meta-analyses reported pooled RDT sensitivity and specificity below the GTFCC minimum surveillance thresholds of 90% and 85%, respectively.
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This systematic review and meta-analysis evaluated the field diagnostic accuracy of cholera RDTs and assessed whether current tests meet the 2024 GTFCC target product profile.

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