Rapid PCR Detects Bloodstream Pathogens in 1 Hour

Multiplex testing showed high agreement with standard microbiological methods in oncology patients.

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The multiplex PCR assay identified bloodstream pathogens in 94% of positive blood cultures within 1 hour.
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Results from the multiplex panel showed high agreement with standard microbiological methods at the genus level.
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The panel detected 96 microorganisms, while standard methods identified 92, with discrepancies mainly at the species level.
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The multiplex panel identified antimicrobial resistance genes in 59 bacteria, with the most common genes being CTX-M and OXA-48–like.
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Limitations of the study included its single-center design, small sample size, and inability to detect organisms not included in the assay.

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