Time-dependent microbiology of peripancreatic drainage fluid in severe acute pancreatitis: a prospective real-world observational study using metagenomic sequencing and culture

1

This study enrolled 20 patients with severe acute pancreatitis undergoing percutaneous catheter drainage for suspected infected pancreatic necrosis.
2

Metagenomic next-generation sequencing (mNGS) identified pathogens in 45% of cases, while conventional culture detected pathogens in 30% of cases.
3

Microbiological positivity rates increased significantly after 14 days from disease onset, highlighting the time-dependent nature of pathogen detection.
4

mNGS revealed a broader spectrum of pathogens, including polymicrobial, anaerobic, and fungal organisms, compared to conventional culture.
5

The findings suggest that early peripancreatic collections are often sterile, while mNGS provides valuable information in later stages of severe acute pancreatitis.

1