Evaluating the Diagnostic Utility of 16S Oxford Nanopore Technology Sequencing in Patients With Central Nervous System Infections and Its Usefulness in Antimicrobial Stewardship - Takeaways - MDSpire

Evaluating the Diagnostic Utility of 16S Oxford Nanopore Technology Sequencing in Patients With Central Nervous System Infections and Its Usefulness in Antimicrobial Stewardship

  • By

  • Do Van Dong

  • Le Thi Kieu Linh

  • Nguyen Thi Tuyet Nga

  • Nghiem Xuan Hoan

  • Nguyen Thi Khanh Linh

  • Tran Thi Thanh Huyen

  • Hoang Xuan Quang

  • Tran Thi Lien

  • Van Dinh Trang

  • Vu Viet Sang

  • Peter G Kremsner

  • Le Huu Song

  • Dennis Nurjadi

  • Thirumalaisamy P Velavan

  • May 26, 2025

  • 0 min

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  • 1

    The study evaluated the diagnostic efficacy of 16S Oxford Nanopore Technology sequencing for CNS infections compared to traditional CSF culture methods.

  • 2

    A total of 329 patients were analyzed, with 40 samples testing positive for pathogens; 16S ONT detected 28, while CSF culture identified 23.

  • 3

    16S ONT sequencing identified 17 pathogens not detected by CSF culture, highlighting its superior sensitivity and diagnostic yield.

  • 4

    Based on 16S ONT findings, 61% of patients received inappropriate empirical antibiotic therapy, indicating a need for improved antimicrobial management.

  • 5

    The study emphasizes the potential of 16S ONT sequencing to enhance antimicrobial stewardship and improve patient outcomes in resource-limited settings.

Original Source(s)

Evaluating the Diagnostic Utility of 16S Oxford Nanopore Technology Sequencing in Patients With Central Nervous System Infections and Its Usefulness in Antimicrobial Stewardship

  • by Do Van Dong, Le Thi Kieu Linh, Nguyen Thi Tuyet Nga, Nghiem Xuan Hoan, Nguyen Thi Khanh Linh, Tran Thi Thanh Huyen, Hoang Xuan Quang, Tran Thi Lien, Van Dinh Trang, Vu Viet Sang, Peter G Kremsner, Le Huu Song, Dennis Nurjadi, Thirumalaisamy P Velavan

  • May 26, 2025

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